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Biosafety
Adeno-Associated Virus (AAV)
Custom-Made AAVs
Custom-Made AAVs
Custom-Made AAVs
BrainVTA offers tailor-made AAVs for the customers and presents a wide variety of options to choose from. All customized services undergo stringent control to guarantee the quality of the viral particles you receive each time.
Key informations
● The packaging capacity is~4.7 kb from ITR to ITR for single stranded AAV and ~2.2 kb from ITR to ITR for self-complementary AAV.
● Viral particles are purified by ultracentrifugation on an iodixanol step gradient.
● Quality control assays include qPCR to quantify viral titers and silver stain to determine viral titer and purity after purification.
● Serotypes include 1, 2, 5, 6, 8, 9, rh10, DJ, DJ8, PHP.B, PHP.eB, PHP.S, retro, anc80. Novel peptide modified serotypes can also be produced on a case-by-case basis. For information on Serotype and Tissue Specificity, please visit introduction to AAV Services.
● Here are some examples of different types of custom-made AAV vectors that have been produced successfully:
-CRISPR/ Cas9
-Optogenetics
-DREADDS
-Calcium sensors
-Cre / FlpO
-Tracing virus helper
-Overexpression
-RNAi
AAV specific infection in some organs and tissues.
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