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CircRNA Service

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CircRNA Service

CircRNA Service

Circular RNA (circRNA) is a type of single-stranded RNA, unlike linear RNA,forms a covalently closed continuous loop, most of them exist in cytoplasm. BrainVTA provides one-stop solution for circRNA over-expression and silence service, including vector construction, virus packaging, cell transfection and qPCR validation.Our most experienced experts will work with you to generate a custom product that best fits your needs.

CircRNA Over-Expression Service

BrainVTA offer pAAV-circRNA, pLV-circRNA and AdV-circRNA over-expression clone and virus package sevice. We has developed an efficient loop-forming
vector system, which can promote linear sequence efficient ring formation, to facility your circRNA research.Please contact us for more information!

CircRNA Knockdown Service

Multiple siRNA sequences will be designed according to the adaptor sequence of circRNA, specific and high efficiency shRNA will be used to clone in the
vector.

MiRNA Services FAQs

1.What are the requirements for sequence length for CircRNA overexpressed?

The best length is in the range of 300-2000bp, otherwise, it is not easy to cyclization.

References

Xiaohui Bai et al. CircNf1-mediated CXCL12 expression in the spinal cord contributes to morphine analgesic tolerance. Brain Behav Immun. 2023.
Jing Wu et al. CircRalgapa1 facilitates morphine tolerance via miR-873a-5p/A20 axis in mice. Neuropharmacology. 2023.

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